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. 2020 Jan 8;20:25. doi: 10.1186/s12885-019-6499-y

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — IFNγ increases PD-L1 expression in osteosarcoma cell lines. Surface markers of human and murine osteosarcoma cell line were evaluated by flow cytometry. The upper row shows representative specimen. In the lower row, each specimen is plotted (n = 3), and the average value is indicated by a horizontal bar. a Expression of IFNGR1 in each cell line. b Expression of PD-L1 in each cell line. Iso-type control, anti-PD-L1 staining with/without IFNγ were evaluated