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. 2020 Jan 8;20:25. doi: 10.1186/s12885-019-6499-y

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Anti-PD-1 antibody improves survival curve in vivo. The LM8 murine osteosarcoma cell line was subcutaneously transplanted to the dorsal region of the C3H mouse, and 4H2 was administered (n = 5). Error bars indicate S.D. a Schema of experimental overview. b Changes in tumour volume with time to first death. The right line graph plots the mean value of the tumour volume. c Evaluation of overall survival rate by Kaplan-Meier curve. P value of log rank method was 0.025