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. 2020 Jan 8;13:9. doi: 10.1186/s13071-020-3883-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Detection of mChPGF2S in highly-infected macrophages. a Detection of mCherry fluorescence in the host cell cytoplasm. Time-lapse images of macrophages infected with more than three mChPGF2S parasites. Cyan was used as pseudocolor; numbers at the bottom right of each panel indicate hour:minutes after infection. b Close image analysis of mCherry and mChPGF2S localized fluorescence intensity in infected macrophages. Numbers at the top of each image indicate hour:minutes after infection. c Overall intracellular fluorescence in infected macrophages was quantified using FIJI (ImageJ) software up to 30 h pi. Scale-bars: a, 10 µm