Fig. 2.
Laminar distribution of Aβ plaques in the hippocampus of an APP knock-in mouse model. a Representative images of 2, 3, 4, and 6-month old APPNL-G-F/NL-G-F mouse hippocampal sections immunostained for VGLUT1 (to distinguish laminae), WFS1 (to distinguish subfields), and 6E10 (for Aβ plaques). b Corresponding masks used to quantify laminar plaque load. c-f Quantification of the Aβ plaque load in hippocampal subfields c and laminae of CA1 d, CA2/3 e, and dentate gyrus (DG) f. Graphs show means ± SEM. (n = 7 mice). SO = stratum oriens; SP = stratum pyramidale; SR = stratum radiatum; SLM = stratum lacunosum-moleculare; MO = molecular layer; SG = stratum granulosum; H = hilus; DG = dentate gyrus