Fig. 4.

NLRC3 suppresses NOD1- and NOD2-induced NF-κB-dependent transcriptional activity. a Luciferase reporter assay for NF-κB activity of NLRC3. b NLRC3 coexpression suppresses NOD1-induced NF-κB-dependent transcription. c NLRC3 coexpression suppresses NOD2-induced NF-κB-dependent transcription. Both NOD1 and NOD2 protein levels did not change when they were cotransfected with NLRC3. Luciferase assays were performed in triplicate, and plates were read at least 3 times. Assays were repeated in 2 independent experiments. Student's t test was used for statistical analysis of the results.