Fig. 5.

NLRC3 negatively regulates IL-1β maturation and caspase-1 cleavage. a NLRC3 inhibits pro-IL-1β protein expression, IL-1β maturation and caspase-1 cleavage. b NLRC3 inhibits NLRP3/cryopyrin inflammasome-induced IL-1β maturation and caspase-1 cleavage. c NLRC3 expression leads to a moderate reduction in extracellular IL-1β levels. In brief, 24 h after transfection, supernatant and transfected cells were collected. Cells were lysed to profile the expression of intracellular IL-1β-p17. Supernatants, on the other hand, were precipitated to test the effect of NLRC3 on IL-1β secretion. Immunoprecipitation was performed with rabbit anti-human IL-1β-p17 antibody, and immunoblotting was performed using rabbit anti-human IL-1β-p17. IP = Immunoprecipitation; WB = Western blotting; x = 250 ng of plasmid.