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. 2014 Aug 13;7(1):37–46. doi: 10.1159/000365112

Fig. 3.

Fig. 3

TM9SF2 and TM9SF4 interact with PGRP-LC. a, bDrosophila S2 cells were transfected with pAc-PGRP-LC-V5 expressing full (a) or truncated (b) forms of the isoform PGRP-LCx and either pAc-TM9SF4-GFP or pAc-TM9SF2-GFP, as indicated. Cell extracts were immunoprecipitated (IP) with either anti-GFP (αGFP) or anti-V5 (αV5) antibodies, separated on SDS-PAGE and Western blotted (WB) with αV5 or αGFP, as indicated. b Recombinant protein expression was assessed by Western blot of cell lysates with αGFP or αV5. Asterisks indicate unspecific bands. c Extracts of flies expressing the Flag-tagged PGRP-LCx isoform with either TM9SF2-GFP or TM9SF4-GFP were immunoprecipitated with an anti-GFP antibody. Co-immunoprecipitated product was revealed on a Western blot with anti-Flag (αFlag) antibody (upper panel). Expression of TM9-GFP fusion proteins was assessed by Western blotting of fly lysates with anti-GFP antibody (αGFP; bottom panel). Genotypes are as follows: w1118;dagal4/+ (daughterless-gal4), w1118;​daGal4/+;PGRP-LCx-Flag#16B, TM9SF2-GFP and w1118;​daGal4/+;PGRP-LCx-Flag#16B, TM9SF4-GFP.