Fig. 4.

Orexin-A (i.pag.)-induced antinociception is antagonized by i.pag. blockade of NK₁Rs or mGlu₅Rs. a-b: Time courses of antinociceptive effects (expressed as % MPE) induced by orexin-A (1.0 nmol, i.pag.) in combination with the vehicle or the antagonist of NK₁Rs (L-703,606, 10 nmol, i.pag., a or mGlu₅Rs (MPEP, 30 nmol, i.pag., b in the mouse hot-plate test. (two-way ANOVA /post hoc Bonferroni test). c: The AUC of the antinociceptive effect in each treatment group (one-way ANOVA /post hoc Tukey test). The antagonist was i.pag. co-administered with i.pag. orexin-A. The data presentation and statistics are the same as in Fig. 2. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the vehicle control group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. the orexin-A group