Fig. 3.

LINC00958 targets miR-3619-5p to exert its tumor-promoting effects in HCC. a RIP assay for AGO2 was conducted to detect the levels of endogenous LINC00958 in the AGO2 IP pellet. b A total of six miRNAs were predicted to harbor complementary sequences to LINC00958 according to starBase and miRDB databases. c AGO2-RIP assays showed the enrichment of the predicted six miRNAs in Hep3B and HepG2 cells with LINC00958 overexpression or NC. d Enrichment of LINC00958 in HCCLM3 and Focus cells transfected with miR-3619-5p mimics or miR-control. e Putative binding sequence between LINC00958 and miR-3619-5p. f Luciferase reporters containing WT or MUT LINC00958 transcript as well as blank pmirGLO were co-transfected with miR-3619-5p mimics or miR-control in HCCLM3 and Focus cells. Luciferase activity was determined using dual luciferase reporter system. g Enrichment of LINC00958 pulled down by biotin-miR-3619-5p or negative control. h FISH results showing the colocalization of LINC00958 and miR-3619-5p in cytoplasm in HCC cells. **P < 0.01, ***P < 0.001