Fig. 5.

LINC00958 positively correlates with lipogenesis in HCC cells. a Cellular cholesterol levels were assessed in HCC cells with LINC00958 knockdown or overexpression. b Cellular levels of triglyceride were assessed in HCC cells with LINC00958 knockdown or overexpression. c Expression levels of SREBP1, FASN, SCD1, and ACC1 in HCCLM3 and Focus cells with LINC00958 knockdown were detected using RT-qPCR. d Expression levels of SREBP1, FASN, SCD1, and ACC1 in Hep3B and HepG2 cells with LINC00958 overexpression were examined using RT-qPCR. e Western blotting was used to determine the levels of SREBP1, FASN, SCD1, and ACC1 in LINC00958-silenced HCCLM3 and Focus cells and LINC00958-overexpressed Hep3B and HepG2 cells. f Oil Red O staining showing the lipid droplets in HCC cells with LINC00958 knockdown or overexpression. g Representative FISH images showing the expression levels of LINC00958 and miR-3619-5p in LINC00958^high and LINC00958^low HCC patient tissues. h Oil Red O staining was performed to show the lipid droplets in tissue samples of LINC00958^high and LINC00958^low HCC patients. **P < 0.01, ***P < 0.001