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. 2019 Dec 14;8(24):e014451. doi: 10.1161/JAHA.119.014451

Table 2.

Vascular and Cardiac Histological Analysis

n=7/Group No CVS GFP No CVS siRNA CVS GFP CVS siRNA
Luminal Circumference, mm 4.967±0.058 5.135±0.058 4.897±0.069 4.893±0.019*
Luminal area, mm2 1.694±0.035 1.786±0.037 1.619±0.037 1.550±0.089*
Media thickness, mm 0.107±0.001 0.108±0.002 0.113±0.001 0.114±0.001* ,
Media:lumen area 0.341±0.009 0.335±0.014 0.381±0.005 0.392±0.024* ,
Adventitia collagen (% area) 66.930±1.320 66.820±0.893 71.49±0.313 70.86±0.659* ,
Adventitia thickness, mm 0.040±0.001 0.037±0.002 0.036±0.002 0.034±0.001
Heart weight, g 1.264±0.018 1.266±0.038 1.321±0.028 1.365±0.025* ,
Heart weight/body weight (×100) 0.335±0.005 0.340±0.009 0.355±0.007 0.366±0.007
Myocyte surface area, μm2 349.930±18.701 352.091±11.664 396.888 ±18.842 406.841±8.527* ,
Myocardial collagen (% area) 0.429±0.013 0.449±0.023 0.479±0.042 0.459±0.016

Histological quantification of vascular and myocardial structure. Dimensions of the lumen and media were quantified from elastin staining. Adventitial size and fibrosis were determined from collagen staining. Myocyte surface area was measured with membrane labeling and cardiac fibrosis was queried via collagen staining. There were no effects of small interfering RNA alone. Chronic variable stress increased media thickness, adventitial collagen, and myocyte size. In contrast, CVS siRNA tissue exhibited alterations in all structural end points assessed except myocardial fibrosis. CVS indicates chronic variable stress; GFP, green fluorescent protein; siRNA, small interfering RNA.

*P<0.05 CVS siRNA vs No CVS siRNA; P<0.05 CVS GFP vs No CVS GFP, P<0.05 CVS siRNA vs No CVS GFP.