Fig. S3.

H₂O₂induces HUVECs apoptosis and ROS production. (A) CCK8 assays were used to assess HUVEC viability with gradient H₂O₂ treatment. Quantification of results is shown as means ± SD (***P < 0.001, one-way ANOVA with Bonferroni's post-hoc test). (B) Flow cytometry was performed to assess HUVEC apoptosis with gradient H₂O₂ treatment. Quantification of results is shown as means ± SD (***P < 0.001, one-way ANOVA with Bonferroni's post-hoc test). (C) Immunofluorescence staining of ROS was performed to assess oxidative stress in HUVECs with gradient H₂O₂ treatment. Quantification of results is shown as means ± SD (***P < 0.001, one-way ANOVA with Bonferroni's post-hoc test).