Fig. 3.

Endothelial/myeloid WWP2 knockout in mice aggravates AngII-induced vascular thickening and fibrosis. (A) H&E and (C) Masson's trichrome staining were carried out to assess vascular thickening and fibrosis of vascular tissue specimens from Tek Cre+;WWP2^FL/FL and Tek Cre-;WWP2^FL/FL mice following NaCl (vehicle) or AngII infusions for 2 weeks. Data (B and D) are means ± SD (n = 9 mice per group; ***P < 0.001, unpaired Student's t-test; ^##P < 0.01, two-way ANOVA with Bonferroni's post-hoc test). (E) Western blot analyses were carried out to assess α-SMA and col-1 expression levels. (F) Quantification of immunoblot results are shown as means ± SD (n = 9 mice per group; ***P < 0.001, unpaired Student's t-test; ^###P < 0.001, two-way ANOVA with Bonferroni's post-hoc test). (G) Western blot analyses were performed to assess Septin4, cleaved PARP1, and cleaved caspase-3 expression levels. (H) Quantification of immunoblot results are shown as means ± SD (n = 9 mice per group; ***P < 0.001, unpaired Student's t-test; ^###P < 0.001, ^##P < 0.01, two-way ANOVA with Bonferroni's post-hoc test).