Skip to main content
. 2014 Sep 23;7(1):102–112. doi: 10.1159/000365972

Fig. 1.

Fig. 1

IRF8-/- mice have significantly lower numbers of Ly6C+ monocytes in peripheral organs. BM, spleen and blood from D0, D3, D5 and D7 WNV-infected WT and IRF8-/- mice were processed for flow-cytometric analysis. Inflammatory monocytes were gated as CD45+ CD115+ CD11b+ Ly6G- Ly6C+ cells (a) in the BM (b), spleen (d) and blood (f). Total numbers of Ly6C+ monocytes in the BM (c), spleen (e) and blood (g) were calculated using flow-cytometric percentages and absolute live cells counts for each organ. Flow-cytometric data shown are means ± SD and represent 3 separate experiments with 4 mice/group. Statistical analysis was conducted using one-way ANOVA and the Tukey-Kramer post hoc test. *** p ≤ 0.001 comparing the numbers of WT and IRF8-/- Ly6C+ monocytes at all time points (not shown). ** p ≤ 0.01, *** p ≤ 0.001 comparing numbers of WT Ly6C+ monocytes in the BM, spleen and blood on D7 p.i. to all other days. * p ≤ 0.05 comparing numbers of WT Ly6C+ monocytes in the spleen on D5 p.i. to all other days. # p ≤ 0.05 IRF8-/- Ly6C+ monocytes on D7 p.i. to all other days.