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. 2014 Sep 23;7(1):102–112. doi: 10.1159/000365972

Fig. 2.

Fig. 2

IRF8-/- mice show significantly reduced Ly6C+ macrophage infiltration into the WNV-infected brain. Brains from D0, D3, D5 and D7 WNV-infected WT and IRF8-/- mice were processed for flow-cytometric analysis. CD45+ CD115+ CD11b+ Ly6G- Ly6C+ inflammatory monocyte-derived macrophages were gated in the brain (a, b). Total Ly6C+ macrophage numbers were calculated using flow-cytometric percentages and absolute live cells counts for each brain (c). Immunohistology was also performed on brain sections from D0 and D7 WNV-infected WT and IRF8-/- mice on D7 p.i. Sections were stained with CD45 (red; d-g), CD11b (red; h-k) or Ly6C cells (red; l-o) and counterstained with DAPI (blue) and lectin (green). Flow-cytometric data shown are means ± SD and represent 3 separate experiments with 4 mice/group. Statistical analysis was conducted using one-way ANOVA and the Tukey-Kramer post hoc test. *** p ≤ 0.001 comparing the numbers of WT and IRF8-/- Ly6C+ macrophages on D5 and D7 (not shown). *** p ≤ 0.001 in comparing the numbers of WT Ly6C+ macrophages on D5 or D7 p.i. to all other days. # p ≤ 0.05 comparing the numbers of IRF8-/- Ly6C+ macrophages on D7 p.i. to all other days. Immunohistology was performed on 3 entire sagittal brain sections from a minimum of 3 mice/group, conducted twice.