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. 2020 Jan 6;8:e8362. doi: 10.7717/peerj.8362

Figure 6. Characterization of Edit Plasmids rescued from yeast mitochondrial transgenic cells.

Figure 6

(A and B) Restriction analysis of rescued Edit Plasmids. Lane 1, the original Edit Plasmid DNA, Lane 2–6, rescued Edit Plasmids from yeast cells. Plasmids were digested with NcoI and ClaI (A) or NcoI and PstI (B), and separated by 1.0% agarose gel electrophoresis. (C) Semi-quantitative PCR assay of the Edit Plasmid DNA (HS8) after the cross with the wild-type ρ+ strain. Lane 1, first overnight culture; lane 2, second; lane 3, third; and lane 4, fourth overnight culture. (M) 1 kb plus molecular weight marker (New England Biolabs, Ipswich, MA, USA).