Table 1. List of Edit Plasmids for organelle transformation.
See ‘Materials and Methods’ for details.
| Edit Plasmid | Organism | Organelle | Expr cassette 1 promoter for Cas9 | Expr cassette 2 guide RNA |
Donor DNA | Vector backbone |
|---|---|---|---|---|---|---|
| YP5 | Chlamydomonas | chloroplast | psaA | N/A | N/A | pUC19 |
| YP11 | Chlamydomonas | chloroplast | psaA | sgRNA1c & 2c | N/A | pUC19 |
| YP12 | Chlamydomonas | chloroplast | psaA | sgRNA3c & 4c | N/A | pUC19 |
| YP13 | Chlamydomonas | chloroplast | psaA | sgRNA1c & 2c | HR1c:GFPc:HR2c | pUC19 |
| YP14 | Chlamydomonas | chloroplast | psaA | sgRNA3c & 4c | HR3c:GFPc:HR4c | pUC19 |
| YP6 | Chlamydomonas | chloroplast | psbD | N/A | N/A | pUC19 |
| YP19 | Chlamydomonas | chloroplast | psbD | sgRNA1c & 2c | N/A | pUC19 |
| YP20 | Chlamydomonas | chloroplast | psbD | sgRNA3c & 4c | N/A | pUC19 |
| YP21 | Chlamydomonas | chloroplast | psbD | sgRNA1c & 2c | HR1c:GFPc:HR2c | pUC19 |
| YP22 | Chlamydomonas | chloroplast | psbD | sgRNA3c & 4c | HR3c:GFPc:HR4c | pUC19 |
| YP23 | Chlamydomonas | chloroplast | N/A | sgRNA1c & 2c | HR1c:GFPc:HR2c | pUC19 |
| YP24 | Chlamydomonas | chloroplast | N/A | sgRNA3c & 4c | HR3c:GFPc:HR4c | pUC19 |
| YP29 | Chlamydomonas | chloroplast | N/A | sgRNA1c & 2c | HR1c:GFPc:HR2c | pBR322 |
| YP31 | Chlamydomonas | chloroplast | psaA | sgRNA1c & 2c | N/A | pBR322 |
| YP33 | Chlamydomonas | chloroplast | psbD | sgRNA1c & 2c | N/A | pBR322 |
| HS6 | Saccharomyces | mitochondria | N/A | sgRNA1m & 2m | HR1m:GFPm:HR2m | pBR322 |
| HS8 | Saccharomyces | mitochondria | COX2 | sgRNA1m & 2m | HR1m:GFPm:HR2m | pBR322 |