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. 2019 Dec 23;23(1):100798. doi: 10.1016/j.isci.2019.100798

Figure 7.

Figure 7

FCoR-Foxo1-regulated Dnmt3a Recruitment to the Arx Promoter

(A) Bisulfite-sequencing analysis of the UR2 region of the Arx promoter in islets isolated from 20- to 24-week-old control (n = 15, 263 colonies sequenced), βFoxo1 (n = 10, 157 colonies sequenced), FcorKO (n = 15, 274 colonies sequenced), and DKO (n = 10, 158 colonies sequenced).

(B) Quantification of the bisulfite-sequencing data is shown as %DNA methylation. Data represent means ± SEM. *p < 0.05 and **p < 0.005 by one-way ANOVA.

(C) ChIP assay of MIN6 cells transduced with adenoviruses encoding LacZ (white bar) or HA-ADA-Foxo1 (gray bar) and harvested at 48 h after transduction. Samples were subjected to immunoprecipitation with anti-DNMT1or anti-DNMT3A, followed by PCR amplification of the UR2 region. Data represent means ± SEM from three independent experiments. *p < 0.05 and **p < 0.005 by one-way ANOVA.

(D) ChIP assay of αTC1 cells transduced with adenoviruses encoding LacZ (white bar) or FLAG-FCoR (black bar) and harvested at 48 h after transduction. Samples were subjected to immunoprecipitation with anti-DNMT1or anti-DNMT3A, followed by PCR amplification of the UR2 region. Data represent means ± SEM from three independent experiments. *p < 0.05 and **p < 0.005 by one-way ANOVA.