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. 2019 Dec 19;10:2949. doi: 10.3389/fimmu.2019.02949

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Copyright © 2019 Rinaldi, Consonni, Cordiglieri, Sacco, Crasà, Fontana, Morelli, Elli, Mantegazza and Baggi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Probiotics interact with immune cells located in Peyer's Patches and villi. SIM images of WGA-AF555 (red) labeled-BA (A) and LR (B). (C) Hematoxilin and Eosin staining of rat small intestine. (D) Confocal and (E) SIM images of villi stained for cytokeratin (green) and nuclei (blue). WGA-AF555 LR (red) are located inside villi (confocal image scale bar: 50 μm). (F) Confocal and (G) SIM images of PP stained for vimentin (green) and nuclei (blue). WGA-AF555 LR (red) are located inside the PP (confocal image scale bar: 100 μm). SIM images of CD3⁺ cell (green) (H) and CD11c⁺ cell (green) (I). WGA-AF555 LR (red) are detected nearby CD3⁺ and CD11c⁺ cells. SIM image scale bar: 2 μm.