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. 2019 Dec 19;10:2949. doi: 10.3389/fimmu.2019.02949

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Copyright © 2019 Rinaldi, Consonni, Cordiglieri, Sacco, Crasà, Fontana, Morelli, Elli, Mantegazza and Baggi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Treatment of BMDCs with probiotics modifies R97-116 T cell motility. (A) Experimental set-up of live imaging analyses of CFSE-labeled R97-116-specific T cells co-cultured with R97-116-loaded BMDCs, and example of motility analysis. (B) Percentage, (D) velocity and (F) meandering index of motile Teff. (C) Percentage, (E) velocity, and (G) meandering index of stationary Teff. Experimental conditions were: control cultures of BMDC, loaded with R97-116 peptide (empty bars), BMDC exposed to BBmix prior to antigen loading (black bars), BMDC exposed to TGFβ prior to antigen loading (striped bars). Statistical significance was assessed by two-way ANOVA test with Dunnett's multiple comparison test. Corrected p-values are reported.