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. 2019 Dec 18;10:1587. doi: 10.3389/fpls.2019.01587

Figure 3.

Figure 3

IBM1 modulates the chromatin state of defense marker genes. (A) Detection of H3K9me2 levels. Tissue of 14-day-old seedlings were pooled and chromatin immunoprecipitation was carried out using anti-H3K9me2 antibody for Col-0 and ibm1-4. The associated chromatin was quantified by quantitative PCR with primers spanning across PR1, PR2, and FRK1. Relative enrichments were calculated as percentage of input. Values represent average ± SD from four technical repeats (N = 4). The experiment was repeated twice with similar patterns and one representative repeat is shown. Asterisks indicate significant differences from respective Col-0 wild type controls as determined by a paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01). (B) Detection of H3K4me3 levels for PR1, PR2, and FRK1 were evaluated and analyzed as in A with anti-H3K4me3 antibody.