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. 2019 Dec 27;10:1732. doi: 10.3389/fpls.2019.01732

Table 6.

Detection of X. fastidiosa in environmental plant samples with low population sizes using the tetraplex qPCR assay set n°1 in comparison with the reference test (Harper's test, Harper et al., 2010).

Sample Host plant Place (year) Mean Ct (SEM)a Typing
XF XFFSL XFM XFP Harper's test
1 Centranthus trinervis Bonifaccio, France (2017) nab 33.67 (1.42) na na 34.97 (0.53) Unknown
2 Helichrysum italicum Propriano, France (2017) 27.35 (0.67) na 27.25 (0.23) na 30.85 (0.04) Unknown
3 Lavandula stoechas Vignola, France (2017) 30.75 (0.73) na 26.27 (0.38) na 29.50 (0.13) Unknown
4 L. stoechas Propriano, France (2017) na na na na 34.81 (1.40) Unknown
5 Olea europaea Afa, France (2017) na na na na 34.01 (0.77) Unknown
6 O. europaea Vignola, France (2017) na 29.91 (0.80) na na 32.94 (0.18) Unknown
7 Phylirea angustifolia Bonifaccio, France (2017) na 30.52 (0.21) na na 33.99 (1.09) Unknown
8 Polygala myrtifolia Vignola, France (2017) 24.86 (0.04) na 25.00 (0.03) na 25.96 (0.04) Suspected Xfmc
leuA: 3
9 P. myrtifolia Porto-Vecchio, France (2018) 30.14 (0.58) na 29.52 (0.17) na 32.82 (0.41) Unknown
10 Spartium junceum Corbara, France (2017) 23.68 (0.17) na 23.97 (0.14) na 24.97 (0.06) Unknown

a: None of these tests was performed by the French national reference laboratory.

b: Not amplified.

c: Typing is suspected when the seven housekeeping genes could not be amplified.