| A. | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Ct value (SEM) for each primer and probe set (target strain code) | ||||||||||||
| DNA concentration | Theoretical number of genome copy.ml−1 | XF (CFBP 7970) | XFF (CFBP 7970) | XFM (CFBP 8416) | XFMO (CFBP 8084) | XFP (CFBP 8402) | XFFSL (CFBP 7970) | XFFSL (CFBP 8084) | Harper's (CFBP 7970) | Harper's (CFBP 8416) | Harper's (CFBP 8084) | Harper's (CFBP 8402) |
| 1 µg.ml−1 | 4×108 | 20.03a (0.08) | 18.47 (0.16) | 19.34 (0.04) | 19.09 (0.03) | 16.64 (0.12) | 18.67 (0.01) | 18.94 (0.04) | 17.82 (0.02) | 17.36 (0.05) | 17.80 (0.04) | 16.58 (0.04) |
| 100 ng.ml−1 | 4×107 | 23.31 (0.10) | 21.88 (0.07) | 22.80 (0.10) | 22.78 (0.10) | 19.63 (0.06) | 22.09 (0.05) | 23.10 (0.08) | 21.45 (0.33) | 21.03 (0.09) | 22.13 (0.34) | 19.23 (0.03) |
| 10 ng.ml−1 | 4×106 | 26.56 (0.03) | 25.49 (0.06) | 26.18 (0.09) | 25.91 (0.07) | 22.93 (0.10) | 26.84 (1.01) | 27.55 (0.06) | 25.88 (0.06) | 25.35 (0.12) | 25.55 (1.55) | 22.76 (0.04) |
| 1 ng.ml−1 | 4×105 | 30.22 (0.19) | 28.65 (0.07) | 29.06 (0.12) | 28.89 (0.08) | 25.95 (0.07) | 28.61 (0.24) | 30.78 (0.04) | 29.98 (0.16)a | 29.02 (0.11) | 29.36 (0.11) | 25.77 (0.15) |
| 100 pg.ml−1 | 4×104 | 33.36 (0.43) | 31.57 (0.18) | 32.42 (0.37) | 32.18 (0.20) | 28.95 (0.08) | 31.82 (0.85) | 33.44 (0.16) | na | na | 32.53 (0.20) | 31.55 (0.16) |
| 10 pg.ml−1 | 4×103 | 36.28 (1.36) | na | 37.37 (0.72) | 36.07 (0.59) | 31.82 (0.59) | 33.86 (3.63) | 38.52 (0.08) | na | na | na | 34.28 (0.73) |
| 1 pg.ml−1 | 4×102 | nab | na | na | na | na | na | na | na | na | na | na |
a
A signal is considered positive when obtained in at least two of the three technical repetitions and the lowest concentration at which a signal is obtained is the limit of detection (LOD).
b
Not detected.