Skip to main content
. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2019 Nov 15;146:324–332. doi: 10.1016/j.freeradbiomed.2019.11.016

Figure 2. Characterization of LPO-catalyzed −SeCN oxidation via mass spectrometry.

Figure 2.

−SCN or −SeCN was oxidized with LPO and hydrogen peroxide and the oxidant product was quantified with the TNB assay. The oxidant was then diluted to 10 μM in PBS and extracted for LC-MS. Negative controls with hydrogen peroxide replaced by water were diluted by an equal amount to the oxidant samples. We extracted high mass accuracy (5 ppm) ion chromatograms for the [M-H]− ions of our target compounds, which corresponded to [SCN]− (blue trace), [OSCN]− (red trace), [SeCN]− (purple trace) and [OSeCN]− (green trace). (A) −SCN and LPO without addition of hydrogen peroxide. (B) −SCN and LPO with addition of hydrogen peroxide. (C) −SeCN and LPO without addition of hydrogen peroxide. (D) −SeCN and LPO with addition of hydrogen peroxide.