Figure 1.

Hypoxia Led to Injury of H9c2 Cells and Upregulated BNIP3 Expression

(A and B) CCK-8 and EdU assays (scale bar = 100 μm) detected that -cell viability of H9c2 cells in hypoxia group was lower than that in normoxia control. (C and D) TUNEL (scale bar = 100 μm) and caspase-3 activity analyses were used to assess the increased apoptosis rate of H9c2 cells under hypoxia versus normoxia control. (E) Western blot analysis determined the higher levels of cleaved caspase-3/9, Bax and lower level of Bcl-2 in H9c2 cells under hypoxia versus normoxia control. (F) Quantitative real-time PCR and western blot analyses were utilized to evaluate the increased level of BNIP3 in H9c2 cells under hypoxia versus normoxia control. Bar graphs were shown as mean ± SD. *p < 0.05, **p < 0.01.