Figure 4.

Histopathology from Matched Treated and Untreated Regions in Study and Uninjected Control Eyes

(A–D) Images represent sequential serial sections taken from inside (Bleb) or outside (Non-bleb) the treatment region. All samples are from the temporal or nasal visual streak, and the corresponding locations are illustrated for study and control eyes. Each image grouping shows H&E-stained sections (left) and immunocytochemistry sections dual labeled with RPE65 and hCAR (middle) or RPE65 and rod opsin (right); DAPI labels nuclei blue. RPE65 labeling is only present inside the treatment bleb. hCAR labels intensely the cone inner segments and synaptic terminals, and rod opsin the rod outer segments, and, when rod opsin is delocalized, the rod inner segments. Scale bar, 40 μm applies to all sections.