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. 2019 Sep 3;28(1):189–201. doi: 10.1016/j.ymthe.2019.08.016

Figure 6.

Figure 6

KPT-350 Treatment Increases Overall Macrophage Populations in D2-mdx Mice

(A) TSNE plots of flow cytometry data gated on live cells of WT and D2-mdx mice treated with vehicle or KPT-350. For each color, green represents eosinophils (CD11b+F4/80+Siglec-F+), blue represents M1-like macrophages (CD11b+F4/80+Siglec-F−Ly6c+CD206−), and red represents M2-like macrophages (CD11b+F4/80+Siglec-F−Ly6c−CD206+) populations. Eight-week-old WT and D2-mdx male mice were given oral KPT-350 three times a week for 8 weeks. Single-cell suspensions were prepared from quadriceps and tibialis anterior muscles for analysis via flow cytometry. (B–E) Quantification of flow cytometry analysis showing the frequency (of total cells) of eosinophils (B), total macrophages (C), M1-like (D), or M2-like (E) macrophages. (F and G) Mean fluorescence intensity (MFI) of PD-L1 in M1-like (F) or M2-like (G) macrophages. (H) F4/80 staining in the tibialis anterior muscle of the four experimental cohorts. Scale bars, 500 μm. Mean ± SEM. n = 5. *p < 0.05, two-way ANOVA with a Tukey correction.