Skip to main content
. Author manuscript; available in PMC: 2020 Jan 9.
Published in final edited form as: Virology. 2018 Mar 30;518:369–376. doi: 10.1016/j.virol.2018.03.007

Figure 3.

Figure 3

ADK6 binding and neutralization assays. A) Immunoblot of denatured WT and variant AAV1 and AAV6 vectors detected by B1 (which recognizes a linear epitope of the C-terminus of the VP1/2/3 common region) (top) and by ADK6 (which recognizes native capsids). ADK6 interacts specifically with AAV6, and this interaction is lost in mutant AAV6-K531E and restored in AAV1-E531K. B and C) Neutralization assays for WT and variants of AAV1 and AAV6, respectively, in the presence of ADK6. Transduction is normalized to the luciferase signal for WT virus at zero antibody concentration.