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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Biochem Pharmacol. 2019 Nov 21;171:113727. doi: 10.1016/j.bcp.2019.113727

Figure 7.

Figure 7.

Effects of G-1 and G-36 on ISO-stimulated phosphorylation of Cav1.2. A and B, Verification of anti-phospho Ser1928 antibody. HEK293 cells were transfected with mock conditions or plasmids encoding EYFP-CaV1.2(1887-1905) with wild-type or R1894A/R1895A/S1897A substitutions as indicated. Cells were treated for 10 min with or without 10 μM ISO as specified in medium containing 1.5 mM CaCl2, followed by lysis. Upper panels, Ponceau-S stains. Lower panels, corresponding immunoblots using the anti-phospho Ser1928 antibody (PA5-64748, left) or anti-GFP antibody (ab6673, right). Freshly isolated cardiomyocytes were pre-treated with the specified doses of G-1 (B) or G-36 (C) for 15 minutes followed by 1 μM ISO treatment for 10 minutes, lysis, and immunoblotting. Following SDS-PAGE, membranes were stained with Ponceau-S followed by washing and probing with anti-PSer1928 Cav1.2 antibody (upper blots). Histograms, relative densitometric values of P-Ser1928/total protein loading. ****, p < 0.0001 vs control; n = 6 for each condition.