Fig 1. DEHP dose-response curve, plate phenotyping analysis and structures and representative GC-MS and HPLC-MS/MS chromatograms for DEHP and its metabolites.

(A) Quantification of germ cell apoptosis, which takes place in late pachytene, from worms exposed to a range of DEHP concentrations compared to vehicle alone (0.1% DMSO). Analysis was done for three independent biological repeats, and more than 25 gonads were scored for each indicated exposure. Error bars represent SEM. **P<0.01, ***P<0.001 and ****P<0.0001 by the two-tailed Mann-Whitney test, 95% C.I. (B) The brood size, embryonic lethality, larval lethality and incidence of males were scored for the progeny laid by hermaphrodites continuously exposed to 10 μM, 100 μM and 500 μM DEHP starting from eggs until 24 hours post-L4 (adulthood) and compared to vehicle alone (0.1% DMSO). Indicated P values were calculated by χ² analysis; n.s. = not significant. (C) Representative gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) chromatograms from the analysis of internal circulating levels of DEHP and its metabolites in worms exposed either to vehicle alone (DMSO) or to DEHP (left) and chemical structures of DEHP and its metabolites (right).