Figure 2.

mir-34a Regulates Macrophage Polarization Partly via LXRα

(A–F) THP-1 cells (A–C) or RAW267.4 cells (D–F) were transfected with 75 nM miR-34a mimics, miR-34a inhibitors, or scramble control oligos (n = 4–6). After 24 h, mRNA levels (A, C, D, and F) or protein levels (B and E) were determined. (G and H) Peritoneal macrophages were isolated from chow-fed miR-34a^+/+ or miR-34a^−/− mice. mRNA (G) and protein (H) levels were determined (n = 3–4). (I and J) Peritoneal macrophages were isolated from chow-fed miR-34a^+/+ or miR-34a^−/− mice and then infected with lentiviruses expressing shRNA against scramble sequences (Lenti-shScr) or Lxrα (Lenti-shLxrα) for 48 h. mRNA levels were determined (n = 3). (K and L) Peritoneal macrophages were isolated from chow-fed miR-34a^+/+ or miR-34a^−/− mice and then treated for 24 h with vehicle, LPS (1 μg/mL) plus IFNγ (50 ng/mL) (K), or IL-4 (20 ng/mL) (L) (n = 4–6). The mRNA levels for M1 (K) or M2 (L) macrophages were quantified. See also Figures S4–S6. All of the data are expressed as mean ± SEM. In (A)–(J), a two-tailed Student’s t test was used for statistical analysis. In (K) and (L), a two-way ANOVA test was used for statistical analysis. *p < 0.05, **p < 0.01.