Figure 4.

Global Loss of miR-34a Improves Hyperlipidemia and Attenuates the Development of Atherosclerosis in Ldlr^−/− Mice

(A–H) 34a^+/+Ldlr^−/− or 34a^−/−Ldlr^−/− mice were fed a Western diet for 18 weeks. Plasma cholesterol (A) or TG (B) levels were determined (n = 14–15). Plasma cholesterol (C) or TG (D) lipoprotein profiles were analyzed by fast protein liquid chromatography (FPLC). En face aortas were stained by oil red O (ORO) (E), and the plaque size was determined (n = 12) (F). Aortic roots were also stained by ORO (G), and the plaque size was determined (n = 9–10) (H). See also Figures S10 and S11. All of the data are expressed as mean ± SEM. A two-tailed Student’s t test was used for statistical analysis. *p < 0.05, **p < 0.01.