mRNA Analysis in Microglia after Incubation with IL-4 or GM-CSF
Analysis of mRNA gene expression in cultured microglia in CTR (no cytokine buffer control), IL-4 (20 [IL4-20] or 40 [IL4-40] ng/mL), or GM-CSF (20 [GM-20] or 40 [GM-40] ng/mL) on day 3. Bar graphs show the relative mRNA expression of Cd86, Mrc1, Nos2, and Arg1 in microglia against the CTR group. These mRNA expressions were normalized to that of β-actin. Error bars showed mean + SEM (n = 3 in each group). *p < 0.05 as compared with the CTR group.