Figure 5.
Schematic Procedures and Motor Function at M2 Microglia Transplantation Therapy in the SCI Mouse Model
(A) Schematic procedures of mouse SCI and M1 or M2 microglia transplantation therapy. After exposing the spinal cord (upper middle image), 1-mm circle and 1-mm-depth injury was made at the right side of the mouse spinal cord by a 1-mm-diameter skin biopsy puncher (right panel). A total of 1.0 × 105 M1 or M2 microglia cells (incubated with GM-CSF or IL-4) were transplanted with Matrigel at 3 μL volume into the injured spinal cord (right panel). (B) Time course of the Basso mouse scale in SCI mice after transplantation of Matrigel only (CTR) or M1 or M2 microglia with Matrigel from pre-injury (Pre) to day 28 after injury. Bars showed means ± SEM (n = 9–15 in each group). (C) Time course of the hindlimb reflex scoring in SCI mice after transplantation of Matrigel only (CTR) or M1 or M2 microglia with Matrigel from pre-injury (Pre) to day 28 after injury. Bars showed means ± SEM (n = 5 in each group). (D and E) Time course of the Basso mouse scale (D) and time course of the hindlimb reflex scoring (E) in SCI mice after transplantation of M2 microglia on day 0, 3, or 7 after injury. Bars showed means ± SEM (n = 5–7 in each group). (F and G) Time course of the Basso mouse scale (F) and time course of the hindlimb reflex scoring (G) in SCI mice after transplantation of M2 microglia or in that with systemic injection therapy of IL-4 from pre-injury (Pre) to day 28 after injury. Bars showed means ± SEM (n = 5–7 in each group). *p < 0.05 compared with other groups by two-way ANOVA and Scheffe’s test. CTR, no cell administered (Matrigel only) group.