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. 2019 Sep 10;28(1):254–265. doi: 10.1016/j.ymthe.2019.09.004

Figure 6.

Figure 6

Histological Analysis of Spinal Cords after Injury and Cell Transplantation in SCI Mice

(A) Immunohistochemistry of spinal cord with β3-tubulin (as a neuron marker: red), GFAP (as an astrocyte marker: red), or Iba-1 antibody (as a microglia marker: red) were shown in Matrigel only (CTR) or M1 or M2 microglia with the Matrigel group 4 weeks after SCI and cell transplantation therapy. Blue showed DAPI stain in nuclei. The broken line areas showed the SCI and the transplanted lesion. (B) Each graph showed the relative fluorescence intensity of β3-tubulin, GFAP, or Iba-1 staining within the transplanted area in M1 or M2 group mice against in the CTR group. Error bars showed means + SD (n = 3 in each group). *p < 0.05 compared with the CTR group. (C) Immunohistochemistry of spinal cord with CD86 (as an M1 microglia marker: red) or CD206 antibody (as an M2 microglia marker: red) was shown in Matrigel only (CTR) or M1 or M2 microglia with Matrigel group 4 weeks after SCI and cell transplantation therapy. Blue showed DAPI stain in nuclei. The broken line areas showed the SCI and the transplanted lesion. Graph showed the relative fluorescence intensity of CD86 or CD206 staining within the transplanted area in the M1 or M2 group against in the CTR group. Error bars showed means + SD (n = 3 in each group). Scale bar, 100 μm. *p < 0.05 compared with other groups. n.s., not significant.