FIG 4.
Verification of the directly binding of CpxR-P to the yoaE promoter regions by EMSA. Lanes 1 to 4, FAM-labeled yoaE Promoter (PyoaE-FAM) incubated with different amounts of phosphorylated CpxR (CpxR-P, 0, 0.5, 1.0, and 2 μg); lanes 5 and 6, FAM-labeled cpxP promoter (PcpxP-FAM) incubated without (lane 5) or with (lane 6) CpxR-P, with PcpxP-FAM used as a positive control; lane 7, 50× unlabeled yoaE promoter probe (PyoaE) competed with PyoaE-FAM. For every lane, 0.5 μg of poly(dI-dC) was added to decrease nonspecific binding.