FIG 5.

Identification of phosphorylated CpxR binding sequence in yoaE promoter and alignment analysis in different species. (A) DNase I footprinting assays of phosphorylated CpxR binding sequence on yoaE promoter region. A 250-bp FAM-labeled yoaE promoter DNA fragment was incubated without or with 2 μg pf phosphorylated CpxR (CpxR-P) and then subjected to DNase I digestion and fragment length analysis. The fluorescence signal of the labeled DNA fragments is plotted against the sequence of the fragment. The sequence of protected region bound by CpxR-P is shown. (B) Alignment of CpxR binding site on yoaE promoter region in the investigated species.