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SloABC and MntH are required for S. mutans tolerance of calprotectin. Data represent growth of UA159 and its derivatives in the presence of purified calprotectin (CP). Overnight cultures were diluted 1:20 into BHI agar, grown to early log phase (OD600 = 0.25), and then diluted 1:50 in CP medium containing (A) no CP, (B) 150 μg ml−1 CP, (C) 200 μg ml−1 CP, or (D) 200 μg ml−1 CP plus 20 μM Mn. The graphs show averages and standard deviations of results from three independent cultures.
