TABLE 4.
Primers used in this study
| Primer | Sequence (5′–3′)a | Application |
|---|---|---|
| smu770Arm1F | GGTCTTAGGGACAAGAGTTAAACGC | mntH deletion |
| smu770Arm1R | CCACTGTATTAACAAGCTTCAACTTGC | mntH deletion |
| smu770Arm2F | CCTCGCTGAGTGAATTCTTTTTTGG | mntH deletion |
| smu770Arm2R | CTGCAAATTTTAAGACTAACTCTTTTATTGGC | mntH deletion |
| sloCArm1F | GATCACGTTCTGCTTTTG | sloC deletion |
| sloCArm1R | GTAATAATAAGCTTAGCATGCTCATTAG | sloC deletion |
| sloCArm2F | GGTTGTTTCGCATGCTTCTCTTAAG | sloC deletion |
| sloCArm2R | GATGCTGTTCCATATAC | sloC deletion |
| smu770comp5’ | CGGGGTACCGAGGATGAAGAGCTTTAATCC | mntH complementation |
| smu770comp3’ | CCGCTCGAGCCTTCATAGATGAACTTACTGC | mntH complementation |
| sloCcomp5’ | CGCGGATCCCAGCGGGTTCAAGCATTGTCTTA | sloC complementation |
| sloCcomp3’ | CCGCTCGAGGGGAGTAAGCGGAAACCTTTCC | sloC complementation |
| sloA.qRT.F | CGTATGCTCTTGGCTCGTTG | qRT-PCR |
| sloA.qRT.R | ACTCCCATCTCAGTTACACCCT | qRT-PCR |
| mntH.qRT.F | AATGCCCAGTTTACCAGCCA | qRT-PCR |
| mntH.qRT.R | TCAGCGAGGTCAATCAGAGC | qRT-PCR |
| mntH_EMSA_F | CTTTTCGCAATCTGATTGTTTAG | EMSA |
| mntH_EMSA_R | CATTTTGAAAATCTCTTTTCTAATATAATTG | EMSA |
a
Restriction sites used to facilitate cloning are indicated in bold.