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Three routes to cointegrate formation between two molecules. (A) Copy-in route; (B) targeted conservative route; (C) homologous recombination. IS26 is indicated by yellow ovals, with orientation shown by “>.” A target site and subsequent 8-bp duplications are indicated by a vertical flag. The relative frequencies of the Tnp26-mediated and homologous-recombination-mediated reactions are indicated by the thicknesses of the arrows.
