FIG 4.

Cointegrate formation between pRMH1010 (IS1216) and R388. The R388 backbone is drawn to scale from GenBank accession no. BR000038 with key resistance genes, genes involved in replication (repA), and genes involved in conjugative transfer (tra) shown as arrows inside the circular backbone. Arrows pointing toward the circular backbone indicate the location of 15 mapped R388::pRMH1010 cointegrates, and the sequence of the 8-bp duplication of the target is shown. Blue lettering indicates that the cointegrate was in orientation 1 (tnp1216 is in the same orientation as the R388 repA gene), and red lettering indicates that the cointegrate was in orientation 2 (tnp1216 is in the orientation opposite to that of R388 repA).