TABLE 2.

Locations of cointegrates formed between pRMH1009 (IS257R2) and E. coli MM383 chromosome

Cointegrate	TSD locationa	TSD sequence	Orientationb
1	1056978–1056985	ATGGGGGA	1
2	2168492–2168499	TGCCACTG	1
3	1504489–1504496	CAGTGGGT	2
4	4325421–4325428	GGCAAGAT	1
5	3569123–3569130	ATACGACG	2
6	3421503–3421510	CTAACTGG	2
7	2966437–2966444	ACGGAGAT	2
8	37426–37433	TTGAGTGG	1
9	4412738–4412745	TGCTACTA	2
10	203519–203526	GAAGAACT	1
11	3308058–3308065	TCGGATTT	1
12	549300–549307	TGATCGCA	2
13	1852645–1852652	CAACGACA	2
14	3931278–3931285	CTAAGCAC	1
15	4002153–4002160	CGCCAATG	2

^aLocation in the E. coli K-12 reference sequence (GenBank accession number U00096.3).

^bOrientation 1 is defined as that of the tnp257 of IS257R2, which is in the same orientation as the positive strand of the K-12 chromosome. Orientation 2 is defined as that of the tnp257 of IS257R2, which is in the orientation opposite to that of the positive strand of the K-12 chromosome.