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. 2020 Jan 8;5(1):e00811-19. doi: 10.1128/mSphere.00811-19

TABLE 5.

Plasmids used in this study

Plasmid Description Inserta Resistance phenotypeb Reference
pRMH1008 IS257-3 in pUC19c Bases 45556–100 from pSK41 Ap This study
pRMH1009 IS257R2 in pUC19c Bases 22809–23851 from pSK41 Ap This study
pRMH1010 IS1216 in pUC19c Bases 25151–26035 from pJEG040 Ap This study
R388 IncW plasmid Su Tp 33
R388::IS257-3 IS257-3 in R388d Bases 45556–100 from pSK41 Su Tp This study
R388::IS257R2 IS257R2 in R388d Bases 22809–23851 from pSK41 Su Tp This study
R388::IS1216 IS1216 in R388d Bases 25151–26035 from pJEG040 Su Tp This study
a

pSK41, GenBank accession no. AF051917; pJEG040, GenBank accession number KX810025.

b

Ap, ampicillin; Su, sulfamethoxazole; Tp, trimethoprim.

c

The insert was cloned into the pUC19 BamHI site by Gibson assembly.

d

The insert was cloned into the R388 HindIII site by Gibson assembly.