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qRT-PCR analysis of DNA damage response genes in N/Tert-1, N/Tert-1+HPV16, and mutant HPV16 genome-containing cell lines. DNase-treated RNA was subject to SYBR green qRT-PCR analysis, and the ΔΔCT was calculated using the GAPDH housekeeping gene and normalized to that for N/Tert-1. Error bars represent the standard error of the mean of three individual experiments. *, P < 0.05, compared to results for parental N/Tert-1 cells.
