TABLE 2.
Calculated fractional occupancy of β-lactamases at various concentrations of BLIPK74T/W112D
| β-Lactamase | Fractional occupancy (f) vs concn of BLIP mutanta
: |
||||
|---|---|---|---|---|---|
| 10 nM | 50 nM | 200 nM | 1,000 nM | 2,850 nM | |
| KPC-2 | 0.806 | 0.952 | 0.988 | 0.998 | 0.999 |
| CTX-M-14 | 0.011 | 0.052 | 0.182 | 0.524 | 0.758 |
| CTX-M-15 | 0.023 | 0.103 | 0.313 | 0.699 | 0.870 |
| TEM-1 | 0.002 | 0.008 | 0.031 | 0.139 | 0.313 |
Fractional occupancy (f) is the fraction of β-lactamase bound at each listed concentration of the BLIPK74T/W112D mutant. The equation for determining f is provided in Materials and Methods.