TABLE 1.

Deduced functions of the open reading frames in the prf gene cluster^a

Protein	No. of aa	Protein function	Homolog, origin	Identity (%), similarity (%)	GenBank accession no.
PrfA	396	MFS transporter	NbrT6, Nocardia terpenica	72, 84	AJO72759
PrfB	476	Adenylosuccinate lyase	Orf67, Nocardia terpenica	81, 89	AJO72760
PrfF	224	HAD family hydrolase	CLW14_2649, Streptomyces sp. strain 75	56, 66	REE32254
PrfH	118	Cupin domain-containing protein	CRH09_08295, Nocardia terpenica	89, 94	ATL66197
PrfTb	334	β-RFA-P synthase-like enzyme	Orf61, Nocardia terpenica	81, 87	AJO72754
PrfC	343	SAICAR synthetase	Orf62, Nocardia terpenica	76, 84	AJO72755
PrfS	540	Fumarate reductase/succinate dehydrogenase	Orf63, Nocardia terpenica	77, 83	AJO72756
PrfE	329	Putative monooxygenase	Sare_0593, Salinispora arenicola CNS-205	55, 64	ABV96521
PrfD	444	Putative monooxygenase	Orf75, Nocardia terpenica	79, 86	AJO72768
PrfG	288	Transcriptional regulator	NbrR10, Nocardia terpenica	59, 70	AJO72769
PrfR	351	Glycine/d-amino acid oxidase	EV381_1130, Actinopolyspora sp. strain DSM 45956	70, 79	RZU68182
PrfM	413	Phosphoribosylglycinamide synthetase	Orf72, Nocardia terpenica	77, 86	AJO72765
PrfL	390	Saccharopine dehydrogenase	Orf71, Nocardia terpenica	66, 77	AJO72764
PrfKb	437	l-Lysine N6-monooxygenase	Spb38, Streptomyces sp. strain SoC090715LN-17	42, 58	BAW27702
PrfJ	668	Methionine-tRNA ligase	Spb40, Streptomyces sp. SoC090715LN-17	33, 47	BAW27704
Prf1	250	SAICAR synthetase	PenC, S. antibioticus	69, 79	AKA87338
Prf2	233	Short-chain dehydrogenase	PenB, S. antibioticus	66, 79	AKA87339
Prf3	260	Hydrolase	DI270_030585, Microbispora triticiradicis	39, 54	RGA01201
Prf4	288	ATP phosphoribosyltransferase	PenA, S. antibioticus	49, 64	AKA87340
PrfU	402	Phthalate 4,5-dioxygenase	Orf68, Nocardia terpenica	79, 89	AJO72761
PrfQ	387	Amidohydrolase	Orf65, Nocardia terpenica	77, 84	AJO72758
Prf5	404	MFS transporter	D5S19_24520, Amycolatopsis panacis	85, 92	RJQ80812
PrfI	156	Flavin reductase	C8D87_103611, Lechevalieria atacamensis	72, 79	RAS67272
PrfN	169	FMN reductase	C8D88_10484, Lechevalieria deserti	71, 82	PWK86923

^aaa, amino acids; MFS, major facilitator superfamily. SAICAR, phosphoribosylaminoimidazole-succinocarboxamide.

^bFunction confirmed in vitro in this study.