TABLE 3.
PCR primers used in this study
| Primer | Sequence (5′–3′) |
|---|---|
| Ni-lfdsaicar-LF | GGTGGCGGTCAACAACGA |
| Ni-lfdsaicar-LR | GGTGGCGACGAAGAACATCA |
| Ni-lfdsaicar-RF | GTCCATATGGCACTTGACCGACCC |
| Ni-lfdsaicar-RR | GGAATTCAGGAGACGACGGTGGT |
| LfdsaicarF | GTCCATATGCCGGAGACTTTCGAT |
| LfdsaicarR | GGAATTCAGCGCCCCGTGTTCAG |
| forT-cas9-gRNA-F | GATGGGTCAACGAGATCACCTTCACGTTTTAGAG |
| forT-cas9-gRNA-R | CTAGCTCTAAAACGTGAAGGTGATCTCGTTGACC |
| forT-cas9-LF | TCGTCGAAGGCACTAGAAGGCCAGCGGCAGCACGACCTTT |
| forT cas9-LR | TCAGGAGACGACGGTGGTGGCGTCGGGTCGGTCAAGTGCCAT |
| forT-cas9-RF | GCCACCACCGTCGTCTCCTGA |
| forT-cas9-RR | GGTCGATCCCCGCATATAGGCGGTGACGAGTTCCAGGTAGCG |
| forT-cas9-idF | AACTTCCAGCCGTGGTAGATGC |
| forT-cas9-idR | CGGTCTTCATTGCCCTGCTTT |
| prfT-cas9-gRNA-F | GATGGGTCAACGAGCTGACCTTCACGTTTTAGAG |
| prfT-cas9-gRNA-R | CTAGCTCTAAAACGTGAAGGTCAGCTCGTTGACC |
| prfT-cas9-LF | TCGTCGAAGGCACTAGAAGGACGAGAAGTTCCGCACCAG |
| prfT-cas9-LR | TGTTGCGGACCGTGGTGAAGGATTCGCCGTCGAGGCT |
| prfT-cas9-RF | TTCACCACGGTCCGCAACA |
| prfT-cas9-RR | GGTCGATCCCCGCATATAGGCAACGGGTATCGGTTCAGC |
| prfT-cas9-idF | AAGGCGGAGTTCGGGTTC |
| prfT-cas9-idR | CGTGTTCATCGACTGGGAGA |
| forT-28a-exp-F | GCTCTAGAACCCGACTACGGACAGGACC |
| forT-28a-exp-R | GAAGATCTGTAGCCGATGCGGAACGAG |
| forK-28a-exp-F | GAAGATCTGGAGGAGCGGAACAACG |
| forK-28a-exp-R | GGAATTCACGCGGAAGACCACATCG |
| prfT-28a-exp-F | GTCCATATGCAGGGAACCGCGGTG |
| prfT-28a-exp-R | GGAATTCCTACAGCCAGGTCGCGGC |
| prfK-28a-exp-F | GTCCATATGAGCACCGGACACGAC |
| prfK-28a-exp-R | GGAATTCTCAGAACGTCAGCGCGAC |