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. Author manuscript; available in PMC: 2020 Jan 9.
Published in final edited form as: Cell Rep. 2019 Dec 24;29(13):4268–4275.e2. doi: 10.1016/j.celrep.2019.11.095

Figure 1. Validation of a Meiotic Drive System to Generate Sexed Embryos.

Figure 1.

(A) Crossing scheme using SD meiotic drive system to generate sex-enriched pools of embryos expressing PCNA-EGFP. Rsps is a sensitive allele of the Rsp locus. SPSD and SD-72 are strong Sd alleles, and we found the drive to be strongest when the SD alleles were in trans.(B) Percentage of adult animals eclosed of each sex due to crossing schemes in (A). The numbers within the bars represent the total number of animals counted.(C) Staging embryos by nuclear cycle (NC) using the PCNA-EGFP transgene. Zygotic genome activation (ZGA) occurs during NC14.(D) Workflow to sort embryos for small-scale ChIP-seq. We hand sorted between 200 and 351 embryos for each biological replicate. We performed two biological replicates for male and female embryos between NC12 and NC14 and one replicate for NC11. To represent a “late” stage of dosage compensation, we chose ~100 2- to 4-h embryos.