KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-CLAMP antibody | Erica N. Larschan | RRID:AB_2195548 |
| Goat anti-MSL3 serum | Mitzi Kuroda | RRID:AB_2147786 |
| rabbit polyclonal anti-H4K16ac | Millipore-Sigma | 07–329; RRID:AB_310525 |
| polyclonal rabbit IgG | Millipore-Sigma | 12–370; RRID:AB_145841 |
| Critical Commercial Assays | ||
| NEBNext ChIP-seq kit | New England Biolabs | E6240L |
| Deposited Data | ||
| Female embryo CLAMP ChIP-seq | NCBI GEO | GSE119448 |
| Cell culture CLAMP ChIP-seq | NCBI GEO | GSE39271 |
| Male embryo CLAMP ChIP-seq | NCBI GEO | GSE133637 |
| Experimental Models: Organisms/Strains | ||
| +; SD72/CyO | Cynthia Staber, Stowers Institute | N/A |
| 19–3, yw, Rsp[s]-B[s]/Dp(2:y)CB25–4, y+, Rsp[s]B[s]; SPSD/CyO | Bloomington Stock Center | BSC64332 |
| yw; attP2{PCNA-EGFP} | Blythe and Wieschaus, 2016 | N/A |
| Software and Algorithms | ||
| Bowtie2 | Langmead and Salzberg, 2012 | version 2.3.0 |
| Picard MarkDuplicates | Picard Toolkit.2019. Broad Institute, GitHub Repository. http://broadinstitute.github.io/picard/; Broad Institute | version 2.9.2 |
| SAMtools | Li et al., 2009 | version 1.9 |
| MACS2 | Zhang et al., 2008 | version 2.1.1 |
| deepTools | Ramírez et al., 2014 | version 3.1.0 |
| bedtools | Quinlan and Hall, 2010 | version 2.27.1 |
| Intervene | Khan and Mathelier, 2017 | version 0.5.8 |