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. 2020 Jan 7;26(1):35–54. doi: 10.3748/wjg.v26.i1.35

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©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Abdominal paracentesis drainage attenuates myocardial cell apoptosis and expression of apoptosis associated protein in myocardium. A: Representative images (400 × magnification) of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. Cells with green staining in the nuclei were determined to be apoptotic cells; B: Immunoblot of Bax and Bcl-2 protein expression from heart samples; C: Densitometry analysis of Bax and Bcl-2. Data are representative of at least three independent experiments; D: Immunoblot of pro-caspase-3 and cleaved-caspase-3 protein expression from heart samples; E: Densitometry analysis of pro-caspase-3 and cleaved-caspase-3. Data are representative of at least three independent experiments. ^aP < 0.05 vs sham group; ^cP < 0.05 vs severe acute pancreatitis group. TUNEL: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; Bax: Bcl-2 related X protein; Bcl-2: B cell lymphoma/leukemia-2 gene; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; SAP: Severe acute pancreatitis; APD: Abdominal paracentesis drainage.